Growth of Slipper Orchid Paphiopedilum javanicum (Reinw. ex Lindl.) Pfitzer during Acclimatization Stage
DOI:
https://doi.org/10.15575/biodjati.v4i2.5665Keywords:
acclimatization, growth, Paphiopedilum javanicum (Reinw. ex Lindl.) Pfitzer, planlet, survival rateAbstract
 Paphiopedilum javanicum (Reinw. ex Lindl.) Pfitzer known as slipper orchid is an endemic terrestrial orchid from Indonesia. This orchid is threatened with extinction and listed under CITES Appendix I. In-vitro propagation of P. javanicum has been done in Bali Botanic Garden (BBG) for last six years but acclimatization stage has not given satisfied result. The purpose of this study was to know the ef-fect of liquid fertilizer on vegetative growth of P. javanicum planlet during acclimatization stage. Beyonic StarTmik liquid fertilizer ap-plied by five different doses considered as treatment of 0, 10, 20, 30 and 40 mL. They were 10 planlets for each treatment considered as replication. In this research we observe vegetative growth of P. ja-vanicum by quantitative and qualitative parameter. Quantitative data were analysis by ANOVA. Result showed, high percentage of planlet survival was observed of 98% and liquid fertilizer dose for 30 mL gave the optimal vegetative growth of P. javanicum planlet with aver-age height of 2.2 cm and leaves number of 13 pieces. There were no differences on qualitative parameter in all treatment that all leaves color was dark green and leaves tessellation appearance was obviousÂ
References
Chandrasekar, B. R., Ambrose, G & Jaya-balan, N. (2005). Influence of Biofer-tilizers and Nitrogen Source Level on The Growth and Yield of Echinochloa frumentacea (Roxb.) Link. Journal of Agricultural Technology, 1, 223–234.
Chen, Y., Goodale, U. M., Fan, X. & Gao, J. (2015). Asymbiotic Seed Germination and In Vitro Seedling Development of Paphiopedilum spicerianum: An Orchid with An Extremely Small Population in China. Global Ecology and Conserva-tion, 3, 367–378.
Cribb, P. (2000). The Genus Paphiopedilum. Natural History Publications (Borneo).
Hazarika, B. N. (2006). Morpho-physiological Disorders in In Vitro Culture of Plants. Scientia Horticulturae, 108, 105–120.
Hendriyani, E. (2010). Uji Media pada Perke-cambahan Paphiopedilum javanicum (Reinw.) ex Lindl. Pfitzer secara In-Vit-ro. Prosiding Seminar Nasional Hor-tikultura Indonesia: Reorientasi Riset untuk mengoptimalkanProduksi dan Rantai Nilai Hortikultura. Universitas Udayana Denpasar, 25-26 Nopember 2010. pp 780–785.
Hong, P. I., Chen, J. T. & Chang, W. C. (2008). Plant Regeneration via Protocorm-like body Formation and Shoot Multipli-cation from Seed-derived Callus of a Maudiae Type Slipper Orchid. Acta Physiol Plant, 30, 755–759.
José da Silva, M., Franco, H. C. J. & Magal-haes, P. S. G. (2017). Soil & Tillage Research Liquid Fertilizer Application to Ratoon Cane Using a Soil Punching Method. Soil & Tillage Research, 165, 279–285.
Liao, Y., Tsai, Y. & Sun, Y. (2011). In Vitro Shoot Induction and Plant Regeneration from Flower Buds in Paphiopedilum Orchids. In Vitro Cell. Dev. Biol. Plant, 47, 702–709.
Ng, C. Y. & Saleh, N. M. (2011). In Vitro Propagation of Paphiopedilum Orchid through Formation of Protocorm-Like Bodies. Plant Cell Tiss Organ Cult., 105, 193–202.
Nhut, D. T., Trang, P. T. T., Vu, N. H., Thuy, D. T. T., Khiem, D. V., Binh, N. V. & Van, K. T. T. (2005). A Wounding Meth-od and Liquid Culture in Paphiopedi-lum delenatii Propagation. Propagation of Ornamental Plants, 5(3), 158–163.
Rychter, P., Kot, M., Bajer, K., Rogacz, D. & Siskova, A. (2016). Utilization of Starch Films Plasticized with Urea as Fertilizer for Improvement of Plant Growth. Car-bohydrate Polymers, 137, 127–138.
Shin, K., Park, S. & Paek, K. (2014). Physio-logical and Biochemical Changes Dur-ing Acclimatization in a Doritaenopsis Hybrid Cultivated in Different Microen-vironments In Vitro. Environmental and Experimental Botany, 100, 26–33.
Udomdee, W., Wen, P., Chin, S. & Chen, F. (2012). Shoot Multiplication of Paphi-opedilum Orchid through In Vitro Cut-ting Methods. African Journal of Bio-technology, 11(76), 14077–14082.
Warseno, T., Hendriyani, E. & Priyadi, A. (2015). Konservasi dan Propagasi Bul-bophyllum echinolabium J.J.Sm. mel-alui Kultur In-Vitro. Prosiding Ekspose Pembangunan Kebun Raya dan Seminar Konservasi Flora Indonesia Memban-gun Kebun Raya untuk Penyelamatan Keanekaragaman Hayati dan Lingkun-gan Menuju Ekonomi Hijau. Bogor, 25- 26 November 2013. pp 773-783.
Wattanawikkit, P., Bunn, E. & Chayanarit, K. (2011). Effect of Cytokinins (BAP and TDZ) and Auxin (2,4-D ) on Growth and Development of Paphiopedilum callosum. Kasetsart J. (Nat. Sci.), 45, 12–19.
Xiao, Y., Niu, G. & Kozai, T. (2011). Devel-opment and Application of Photoauto-trophic Micropropagation Plant Sys-tem. Plant Cell Tiss Organ Cult., 105, 149–158.
Zeng, S., Wang, J., Wu, K., Teixeira, J.A., Zhang, J. & Duan, J. (2013). In Vitro Propagation of Paphiopedilum hangi-anum Perner & Gruss. Scientia Horti-culturae, 151, 147–156.
Zeng, S., Wu, K., Teixeira, J.A., Zhang, J., Chen, Z. & Xia, N. (2012). Asymbiotic Seed Germination, Seedling Develop-ment and Reintroduction of Paphiope-dilum wardii Sumerh. an Endangered Terrestrial Orchid. Scientia Horticultu-rae, 138, 198–209.
Zhang, Y., Wu, K., Zhang, J., Deng, R. & Duan, J. (2015). Embryo Development in Association with Asymbiotic Seed Germination In Vitro of Paphiopedilum armeniacum S.C. Chen et F.Y. Liu. Na-ture Publishing Group, 5(16356), 1–15.
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