Keefektifan beberapa senyawa kimia sebagai agen penginduksi resistensi tanaman sawi terhadap penyakit bercak daun Curvularia
DOI:
https://doi.org/10.15575/4619Keywords:
asam salisilat, Bion M 1/48, kitin, K2HPO4, vitamin B1Abstract
Penyakit bercak daun Curvularia merupakan penyakit baru pada tanaman sawi. Pengendalian penyakit dengan fungisida sintetik sangat berbahaya karena daun sawi dikonsumsi sebagai sayuran mentah atau pengolahan minimal, untuk itu diperlukan alternatif cara pengendalian yang lebih aman bagi kesehatan seperti menginduksi resistensi tanaman menggunakan bahan kimia. Penelitian ini bertujuan untuk mengetahui keefektifan beberapa bahan penginduksi resistensi seperti asam salisilat, kitin, K2HPO4, dan vitamin B1. Sementara, Bion M1/48, bahan penginduksi komersial, digunakan sebagai pembanding. Bahan penginduksi diaplikasikan melalui cara perendaman benih dan disemprotkan. Pada perlakuan perendaman, benih direndam dalam suspensi bahan penginduksi selama 12 jam, sedangkan pada perlakuan penyemprotan, bahan penginduksi disemprotkan satu kali yaitu dua hari sebelum inokulasi dilakukan. Inokulasi patogen yang digunakan memiliki suspensi konidia kerapatan 5 x 105 konidia ml-1. Penelitian ini menggunakan Rancangan Acak Kelompok dengan empat  ulangan. Pengamatan dilakukan terhadap masa inkubasi dan intensitas penyakit. Hasil pengujian menunjukkan bahwa perendaman benih asam salisilat, kitin dan K2HPO4, mampu menunda kemunculan gejala sakit lebih lama dibandingkan Bion M 1/48. Terhadap intensitas penyakit, asam salisilat, kitin, dan K2HPO4, juga efektif menginduksi resistensi tanaman sawi terhadap penyakit bercak daun Curvularia. Ketiga senyawa kimia ini potensial untuk digunakan sebagai pengganti fungisida sintetis dalam mengendalikan penyakit bercak daun Curvularia pada tanaman sawi.
ABSTRACT
Curvularia leaf spot is a new disease on mustard green (Brassica juncea L.). The diseases control with synthetic fungicide is dangerous since mustard green leaves are freshly consumed or with minimum treatments. Therefore, a safer alternative method must be sought such inducing plant resistance using chemical inducers. This research aimed to evaluate effectiveness of salicylic acid, chitin, K2HPO4, vitamin B1, as resistance inducers. Meanwhile, Bion M 1/48, a commercial inducer, was used as the check treatment. These substances were applied as either seed soaking or spraying. In the soaking treatmet, the seeds were soaked for 12 hours duration, whereas in the spraying treatment, chemicals were applied once, at two days before inoculation with conidial suspension of 5 x 105 conidia ml-1. Experimental plants were arranged in a Randomized Block Design with four replicates. Observation was carried out on incubation period and disease intensity. Results showed that soaking the seed in salicylic acid, chitin and K2HPO4 was able to delay the appearance of symptoms, better than of Bion M 1/48. Towards disease intensity, salicylic acid, chitin, and K2HPO4 were also effective in inducing resistance of mustard green leaves against Curvularia leaf spot. The three substances were potential to be applied as an alternative to synthetic fungicide in managing Curvularia leaf spot on the mustard green.
References
Ahn, I-P., Kim, S., & Lee, Y-H. (2005). Vitamin B1 functions as an activator of plant disease resistance. Plant Physiology 138:1505-1515.
Anhar, A. (1999). Penggunaan Pestisida Oleh Petani di Sentra Produksi Sayuran Bukittinggi. Pusat Penelitian IKIP Padang. http://repository.unp.ac.id/723/1/AZWIR%20ANHAR_101_99.pdf
Arinong, A.R., Rukka, H. & Vibriana, L. (2008). Pertumbuhan dan produksi tanaman sawi dengan pemberian bokashi. Jurnal Agrisistem 8 (2):75-80.
Baloch, M.S., Rajput, N.A., Atiq, M., Rehman, A., Khan, S.M., Naveed, K., Khan, B., Ullah, S., & Baloch, N.M. (2018.) Effect of different plant activators against Rhizoctonia solani causing root rot of chilli. Pakistan Journal of Phytopathology 30(01):45-51.
Boubakri, H., Wahab, M.A., Chong, J., Bertsch, C., Mliki, A. & Gacougnolle, I.S. (2012). Thiamine induced resistance to Plasmopara viticola in grapevine and elicited host-defense responses, including HR like-cell death. Plant Physiology and Biochemistry 57:120-33.
El-Mohamedy, R.S.R., Safeek, M.R., El-Samad, E.E.H., Salama, D.M. & Rizk, F.A. (2017). Field application of plant resistance inducers (PRIs) to control important root rot diseases and improvement growth and yield of green bean (Phaseolus vulgaris L.). Australian Journal of Crop Science 11(05):496-505.
Fayadh, A.H., Al-Maaroof, E.A. & Fattah, A.A. (2013). Induced resistance to wheat yellow rust by chemical inducers. Journal of Biology, Agriculture and Healthcare 3(20):56-64.
French, R.D. & Schultz, D. (2011). Alternaria leaf spot of cabbage. Agrilife Extension Texas AM University. Tersedia di https://agrilifecdn.tamu.edu/amarillo/files/2010/11/AlternariaCabbage2011.pdf
Hafez, Y.M., Soliman, N.K., Saber, M.M., Imbabi, I.A., & Abd-Elaziz, A.S. 2014. Induced resistance against Puccinia triticina, the causal agent of wheat leaf rust by chemical inducers. Egyptian Journal of Biological Pest Control 24: 173-181.
Hanif, A., Suryanto, D. & Nurwahyuni, I. (2012). Pemanfaatan bakteri kitinolitik dalam menghambat pertumbuhan Curvularia sp. penyebab penyakit bercak daun pada tanaman mentimun. Jurnal Saintia Biologi 1(1):1-7.
Jackson, G. (2010). Pacific pests and pathogens – fact sheets. Horticulture Week (http://www.hortweek.com/pest-disease-factsheet-mycosphaerella-brassicicola/plant-health/article/1341919).
Noventa, Osca, C., Siswadi dan K. Triyono. 2015. Pengaruh konsentrasi EM4 dan limbah media tanam jamur tiram terhadap intensitas penyakit bercak daun Alternaria pada tanaman pakchoy (Brassica rapa l). Jurnal Inovasi Pertanian 14(1): 68 – 77.
Sarwar, N., Hayat Zahid CH, M. & Haq, I. (2010). Seed treatments induced systemic resistance in chickpea against fusarium wilt in wilt sick field. Pak. J. Bot., 42(5): 3323 – 3326.
Somaya, T., & El-Sharkawy, H.H.A. (2014). Efficacy of some resistance inducers for controlling wheat rust disease (Puccinia triticina fsp tritici). Egyptian Journal of Biological Pest Control 24: 203-209.
Subrahmanyam, P., Mc Donald, D., Waliyar, F., Reddy, L., Nigam, S.N., Gibbons, R.W., Rao, V.R., Singh, A.K., Pande, S., Reddy, P.M. & Subba Rao, P.V. (1995). Screening Methods and Sources of Resistance to Rust and Late Leaf Spot of Groundnut. Information Bulletin no. 47. Technical Report. International Crops Research Institute for the Semi-Arid Tropics, Patancheru, Andhra Pradesh, India.
Suganda, T & Wulandari, D.Y. (2019). Curvularia sp. jamur patogen baru penyebab penyakit bercak daun pada tanaman sawi. Journal Agrikultura 29(3): 119-124.
Suganda, T. (2001). Penginduksian resistensi tanaman kacang tanah terhadap penyakit karat (Puccinia arachidis Speg.) dengan pengaplikasian asam salisilat, asam asetat etilendiamintetra, kitin asal kulit udang, air perasan daun melati, dan dikaliumhidrogenfosfat. Jurnal Agrikultura 12 (2):83-88.
Triki, E., Trabelsi, I., Amri, M., Nefzi, F., Kharrat, M., & Abbes, Z. (2018). Effect of benzothiadiazole and salicylic acid resistance inducers on Orobanche foetida infestation in Vicia faba. Tunisian Journal of Plant Protection 13 (1): 113-125.
Van Loon, L.C. (1997). Induced resistance in plants and the role of pathogenesis-related proteins. European Journal of Plant Pathology 103:753-765.
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